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NameAstrid Daugherty
Organization or InstitutionFAMU-FSU College of Engineering
TopicAdditive Manufacturing
Title

Biofabrication and Rheological Characterization of Archaeal Hydrogels

Author(s)

Astrid Daugherty1,2, Annie Scutte1,2, Tyler Gregory1,2, Sophie Jermyn1,2, David Quashie Jr.1,2, Jamel Ali1,

Author Institution(s)

1 Department of Chemical and Biomedical Engineering, FAMU-FSU College of Engineering, Tallahassee, Florida, 32310, USA
2 National High Magnetic Field Laboratory, Tallahassee, Florida, 32310, USA

Abstract

Tissue engineering is an emerging field that applies chemistry, physics, and engineering concepts to develop functional tissues for organ regeneration and therapeutic applications. Despite considerable progress towards the biofabrication of clinically relevant tissue models, most current in vitro systems fail to account for the two other domains of life, archaea, and bacteria, which are linked to diseases such as cancer. Archaea encompass a number of prokaryotic cells that have been found to occupy the human microbiome. Communities of commensal archaea are known to inhabit the human body and can be commonly found on skin, in the gut, and within oral cavities. However, still little is known about the characteristics of archaea in these microbiomes and their behavior in 3D microenvironments. Knowledge of their role in modulating human tissue physiology may aid in the development of new in vitro models that more accurately mimic in vivo tissues. Here, we incorporate Halobacterium salinarum into alginate-based hydrogels and determine the effects of these organisms on the viscoelastic properties necessary for future extrusion-based 3D printing. We also evaluate the activity and viability of these halophiles through high-resolution imaging and Live/Dead cytotoxicity assays. Exploring the behavior of archaea in 3D culture environments will enable biofabrication of model systems that incorporate multiple domains of life and aid in developing clinically relevant tissue models with microbiomes for high-throughput drug screening applications.